Tuesday, May 12, 2020

Biology Photosynthesis Lab - 934 Words

Biology lab AIM: To test a leaf for starch APPARATUS/MATERIALS : * bunsen burner, tripod stand, wire gauze(OR 90oC electric water bath OR hot plate) * 250 cm3 beaker * boiling tube * anti- bumping granules * forceps * test tube holder * white tile * leaf to be tested (hibiscus leaves are excellent) * 90% ethanol * iodine/potassium iodide solution PROCEDURE: The video above shows the steps in Testing a Leaf for the Presence of Starch. The Procedure outlined below is slightly different. 1. Remove a green leaf fom a plant that has been exposed to sunlight for a few hours 2. Half-fill a 250cm3 beaker with water. Heat the water until it boils. Keep the water at boiling point. 3. Use the†¦show more content†¦Most photosynthesis takes places in the green parts of plants, in particular the leaves. Photosynthesis involves 2 stages - the Light-dependent Stage and the Light-independent stage. In the light-depedent stage, water is photolyzed by sunlight into Hydrogen and oxgen. Hydrogen enters the second stage, oxgen is gaseous by-product. In the light - independent stage enzymes catalyse the reduction of carbon dioxide by hydrogen. The carbohydrate, glucose is first formed. Glucoe may be immediately used, translocated in the form of fructose or sucrose or stored as starch granules. The overall equation for photosynthesis is: 2. Which organic substance does iodine/potassium iodide solution detect the presence of? What observations will you make if the test is positive? Starch. The iodine solution will turn from brown to blue - black. 3. Explain the significance of boiling the leaf in water. Boiling the leaf in water: * Removes the waxy cuticle which prevents entry of iodine/potassium iodide solution. * Ruptures cell membranes to make starch granules in cytoplasm and chloroplasts accessible to iodine/potassium iodide solutiion. Cell membranes are selectively permeable and do not readily allow the penetration of iodine. * Denatures enzymes, particularly those which convert starch to glucose e.g. diastase. Boiling arrests all chemical reactions, since enzymes which catalyse the reactions areShow MoreRelatedBiology Photosynthesis Lab925 Words   |  4 PagesBiology lab AIM: To test a leaf for starch APPARATUS/MATERIALS  : * bunsen burner, tripod stand, wire gauze(OR 90oC electric water bath OR hot plate) * 250 cm3 beaker * boiling tube * anti- bumping granules * forceps * test tube holder * white tile * leaf to be tested (hibiscus leaves are excellent) * 90% ethanol * iodine/potassium iodide solution PROCEDURE: The video above shows the steps in Testing a Leaf for the Presence of Starch. The Procedure outlined below isRead MoreThe Analysis of Spinach Pigmentation During Photosynthesis (biology Lab Report)1050 Words   |  5 PagesSpinach Pigmentationp During Photosynthesis p University of Alabamap I. 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In light reactions, light is absorbed by chlorophyll in the thylakoid membrane and energizes the electronsRead MoreIB Bio lab report on photosynthesis1509 Words   |  7 PagesThe effect of different coloured lights on the rate of Photosynthesis Objective: To find out which colour of light provides the best consequences for the production of oxygen/ the rate of photosynthesis Background: In photosynthesis, there are two main parts, including light dependent and light-independent reactions. Plants use the energy from light for producing sugar, which is being converted into ATP by cellular respiration. They also are the only organisms that produce oxygen along with glucose

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